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. 2020 Feb 21;9(2):147. doi: 10.3390/pathogens9020147

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Role of SLY-negative S. suis strain 89-1591 components in bmDC-produced IL-1β. (A) IL-1β production by bmDCs following 16 h of infection with 1 × 10⁶ CFU of strain 89-1591 or its capsular polysaccharide-deficient mutant (89-1591∆cpsF). (B) IL-1β production 24 h following activation of wild-type (WT) or TLR2^-/- bmDCs with 30 μg/mL of lipoteichoic acid (LTA) extracts from strain 89-1591 or its lgt-deficient mutant (89-1591Δlgt). Non-stimulated cells served as negative control (C-). (C) IL-1β production by bmDCs following phagosomal delivery of 1 μg of S. suis RNA or DNA. Cells stimulated with elution buffer served as negative control (C-). Data are expressed as mean ± SEM (n = 3). * (p < 0.05) indicates a significant difference with 89-1591-derived LTA and # (p < 0.05) with the negative control.