Figure 4. High dose (25 mg/kg) Rapatar treatment of psPten–/– mice leads to increased Akt activation in the liver and the prostate.

Western blot analysis of whole cell extracts (25 ug protein per lane) prepared from the livers (A) and prostates (B) of individual mice treated for 8 weeks with vehicle (n = 3), 25 mg/kg Rapatar (n = 4) or 5 mg/kg Rapatar (n = 4). Membranes were probed with antibodies detecting activated (phosphorylated) and total S6 and Akt proteins. (C) and (D) Quantitation of ratio of activated to total protein for S6 and Akt in livers and prostates respectively based on Western blot signals. Data for individual animals are shown, with the short horizontal black bar indicating the group mean value.