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. 2002 Nov 13;18(3):209–217. doi: 10.1016/0378-1135(88)90088-0

A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus☆

Peter C Roberts 1, James R Etchison 1, Clifford W Bond 2,∗∗
PMCID: PMC7172778  PMID: 2466364

Abstract

An end point dilution microtitration assay is described that can be used for the titration of both cytopathic and non-cytopathic isolates of bovine virus diarrhoea-mucosal disease virus. Indirect immunofluorescence is used to detect infected MDBK cells in the wells of Terasaki plates. The virus titre is derived from the number of uninfected wells, using the Poisson distribution. The assay is simple, fast and economical. Titres of cytopathic virus determined by the microtitration assay and standard plaque assay are equivalent.

Footnotes

☆

Contribution No. J-2098 from Montana Agricultural Experiment Station.

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