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. 2004 Feb 10;188(1):408–413. doi: 10.1016/0042-6822(92)90775-K

Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R☆

Martin Vey ∗, Michaela Orlich †, Sabine Adler ∗, Hans-Dieter Klenk ∗,2, Rudolf Rott †, Wolfgang Garten ∗
PMCID: PMC7172898  PMID: 1566583

Abstract

The hemagglutinin of influenza virus A/FPV/Rostock/34 (H7) was altered at its multibasic cleavage site by site-directed mutagenesis and assayed for proteolytic activation after expression in CV-1 cells. The results indicated that the cellular protease responsible for activation recognizes the tetrapeptide motif R-X-K/R-R that must be presented in the correct sequence position. Studies on plaque variants of influenza virus A/fowINictoria/75 (H7N7) showed that alteration of the consensus sequence resulted in a loss of pathogenicity for chickens.

Footnotes

☆

Part of this work was presented at the With International Congress of Virology in Berlin 1990.

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