Fig. 5.

Amino acid sequence alignment of the encoded polyproteins for known and proposed marafiviruses and PnMV in the region surrounding the coat protein (CP) amino termini. In (A) the proposed initiator methionine residues for the minor (left) and major (right) marafiviral CPs are boxed, as are invariant amino acids encoded by the marafibox. Underlined amino acids indicate conserved sequence around the proposed LXG[G/A] cleavage recognition site for the encoded papain-like protease. (B) Re-alignment of sequences to highlight the proposed conserved protease recognition sequence (boxed). The conserved LXG[G/A] motif resembles that targeted by cellular deubiquitinating enzymes (Ubiquitin). Proposed initiator methionine residues for the minor and major marafiviral CPs are underlined. Although GSyV and GVQ are the same species, the reported sequences for each isolate are not identical and therefore both are included here.