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. 2002 Nov 12;33(3):261–268. doi: 10.1016/0168-1702(94)90107-4

Virus-like particles in MDCK cells persistently infected with Borna disease virus

Richard W Compans a,∗, Lawrence R Melsen a, Juan Carlos de la Torre b
PMCID: PMC7173123  PMID: 7985412

Abstract

A line of Madin Darby canine kidney (MDCK) cells persistently infected with Borna disease virus was examined by electron microscopy. Thin sections revealed the presence of intracytoplasmic virus-like particles ranging from 50–100 nm in diameter. Nuclei of the infected cells exhibited accumulation of electron-dense granular structures 15–18 nm in diameter. The intracytoplasmic particles were roughly spherical with a limiting membrane, suggesting the presence of a lipid-containing envelope. The internal structure consisted of strand-like material which in some cases was condensed underneath the envelope. The possible relationship of these particles to Boma disease virions is discussed.

Keywords: Madin Darby canine kidney cell, Borna disease

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