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. 2004 Sep 17;80(3):397–408. doi: 10.1016/0041-008X(85)90384-9

Increased susceptibility to mouse hepatitis virus 3 of peritoneal macrophages exposed to dieldrin☆

Krzystof Krzystyniak 1,2, Patrice Hugo 1, Denis Flipo 1, Michel Fournier 1
PMCID: PMC7173191  PMID: 2994259

Abstract

Interaction of a single dose (36 mg/kg body wt) of the organochlorine pesticide dieldrin with mouse peritoneal macrophages was examined in C57Bl6, (C57Bl6 × AJ)F1, and AJ strains of different genetic resistance to mouse hepatitis virus 3 (MHV3) infection. In vivo studies showed increased susceptibility to MHV3 acute disease of C57Bl6 and (C57Bl6 × AJ)F1 animals challenged with the pesticide. Significant decrease of mean time of death in dieldrin-exposed, MHV3-infected susceptible C57Bl6 mice was observed similarly upon po or ip administration of a single, sublethal dose of dieldrin. In addition, decrease of humoral response to the virus was quantified by determination of anti-MHV3 IgG antibodies in spleen cell supernatant fractions and in blood sera of dieldrin-exposed C57Bl6 mice. A single dose of dieldrin did not alter the in vivo resistance of AJ animals to acute MHV3 disease. The resistant AJ mice, however, showed increased mortality upon two subsequent exposures to dieldrin followed by infection with high lethal doses of MHV3. Phagocytic activity, cell adherence capacity, and attachment and uptake of 3H-radiolabeled MHV3 by C57Bl6 peritoneal macrophages were determined by in vitro studies. These affector activities of peritoneal macrophages were slightly decreased or unchanged in cells originating from animals exposed to the pesticide. However, the intrinsic activity of MHV3 restriction appeared to be affected in macrophages derived from dieldrin-treated animals: (i) peritoneal C57Bl6 macrophages collected from the early phase of acute MHV3 disease contained increased MHV3 antigen and (ii) increased cytolysis was observed after in vitro MHV3 infection of macrophages originating from dieldrin-exposed C57Bl6 mice.

Footnotes

☆

Supported by Natural Sciences and Engineering Research Council of Canada.

References

  1. Allison A. Lysosomes in virus infected cells. Perspect. Virol. 1967;5:29–41. [Google Scholar]
  2. Antunes-Madeira M.C., Madeira V.M.C. Interaction of insecticides with lipid membranes. Biochim. Biophys. Acta. 1979;550:384–392. doi: 10.1016/0005-2736(79)90143-3. [DOI] [PubMed] [Google Scholar]
  3. Arnheiter H., Baechi T., Haller O. Adult mouse hepatocytes in primary monolayer culture express resistance to mouse hepatitis virus type 3. J. Immunol. 1982;129:1275–1281. [PubMed] [Google Scholar]
  4. Bang F.B., Warwick A. Proc. Natl. Acad. Sci. USA. 1960. Mouse macrophages as host cells for the mouse hepatitis virus and the genetic basis of their susceptibility; pp. 1065–1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bradley S.G., Morahan P.S. Approaches to assessing host resistance. Environ. Health Perspect. 1982;43:61–69. doi: 10.1289/ehp.824361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Casto B., Pieczyǹski W.J., Di Paolo J.A. Enhancement of adenovirus transformation by treatment of hamster embryo cells with diverse chemical carcinogens. Cancer Res. 1974;34:72–78. [PubMed] [Google Scholar]
  7. Crocker J.F.S., Ozere R.L., Safe S.H., Digout S.C., Rozee K.R., Hutzinger O. Lethal interaction of ubiquitous insecticide carriers with virus. Science (Washington, D.C.) 1976;192:1351–1353. doi: 10.1126/science.179146. [DOI] [PubMed] [Google Scholar]
  8. Desaiah D. Interaction of chlorodecone with biological membranes. J. Toxicol. Environ. Health. 1981;8:719–730. doi: 10.1080/15287398109530108. [DOI] [PubMed] [Google Scholar]
  9. Dulbecco R., Vogt M. Plaque formation and isolation of pure line with polyomyelitis viruses. J. Exp. Med. 1954;99:167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dupuy C., Lafforet-Cresteil D., Dupuy J.M. Genetic study of MHV3 infection in mice: In vitro replication of virus in macrophages. In: Skamene E., Kongshavn P.A.L., Landy M., editors. Genetic Control of Natural resistance to Infection and Malignancy. Academic Press; New York/London: 1980. pp. 241–246. [Google Scholar]
  11. Dupuy J.M., Rodrique D. Heterogeneity in evolutive patterns of inbred mice infected with a cloned substrain of mouse hepatitis virus type 3. Intervirology. 1981;16:114–117. doi: 10.1159/000149255. [DOI] [PubMed] [Google Scholar]
  12. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen coated tube. J. Immunol. 1972;109:129–135. [PubMed] [Google Scholar]
  13. Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal. Biochem. 1982;119:142–154. doi: 10.1016/0003-2697(82)90677-7. [DOI] [PubMed] [Google Scholar]
  14. Hodge H.C., Boyce A.M., Deichmann W.B., Kraybill H.F. Toxicology and no-effect levels of aldrin and dieldrin. Toxicol. Appl. Pharmacol. 1967;10:613–675. doi: 10.1016/0041-008x(67)90100-7. [DOI] [PubMed] [Google Scholar]
  15. Howett M.K., Pegg A.E., Rapp F. Enchancement of biochemical transformation of mammalian cells by herpes simplex virus following nitrosomethylurea treatment. Cancer Res. 1979;39:1041–1045. [PubMed] [Google Scholar]
  16. Ito M., Ralph P., Moore M.A.S. In vitro stimulation of phagocytosis in a macrophage cell line measured by a convenient radiolabelled latex bead assay. Cell. Immunol. 1979;46:48–56. doi: 10.1016/0008-8749(79)90244-2. [DOI] [PubMed] [Google Scholar]
  17. Kaminski N.E., Roberts J.F., Guthrie E.F. The effects of DDT and dieldrin on rat peritoneal macrophages. Pest. Biochem. Physiol. 1982;17:191–195. [Google Scholar]
  18. Kern E.R. Use of viral infections in animal models to assess changes in the immune system. Environ. Health Perspect. 1982;43:71–79. doi: 10.1289/ehp.824371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Krzystyniak K., Dupuy J.M. Early interaction between mouse hepatitis virus 3 and cells. J. Gen. Virol. 1981;57:53–61. doi: 10.1099/0022-1317-57-1-53. [DOI] [PubMed] [Google Scholar]
  20. Krzystyniak K., Dupuy J.M. Immunodepression of lymphocyte response in mouse hepatitis virus 3 infection. Biomed. Pharmacother. 1983;37:68–74. [PubMed] [Google Scholar]
  21. Krzystyniak K., Dupuy J.M. Entry of mouse hepatitis virus 3 into cells. J. Gen. Virol. 1984;65:227–231. doi: 10.1099/0022-1317-65-1-227. [DOI] [PubMed] [Google Scholar]
  22. Krzystyniak K., Roy D., Flipo D., Fournier M. Pesticide-induced immunodepression and alteration of antiviral natural resistance in mouse hepatitis virus 3 infection. International Seminar on the Immunological System as a Target for Toxic Damage; Luxembourg, 6–9 November; 1984. pp. 95–99. [Google Scholar]
  23. Lai M.M., Stohlman S.A. RNA of mouse hepatitis virus. J. Virol. 1978;26:236–242. doi: 10.1128/jvi.26.2.236-242.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Lamontagne L., Dupuy J.M. Persistent in vitro infection with MHV3. 25th Annual Meeting of the Can. Fed. Biol. Soc.; Edmonton; 1982. p. 168. abstract No. 664. [Google Scholar]
  25. Lamontagne L., Dupuy J.M. Persistent in vitro infection with mouse hepatitis virus 3. In: Rottier P.J.M., van der Zeijst B.A.M., Spaan W.J.M., Horzinek M., editors. Molecular Biology and Pathogenesis of Coronaviruses. Plenum; Amsterdam/London: 1984. pp. 315–325. [Google Scholar]
  26. Levy-Leblond E., Oth D., Dupuy J.M. Genetic study of mouse sensitivity to MHV3 infection: Influence of the H-2 complex. J. Immunol. 1979;122:1359–1362. [PubMed] [Google Scholar]
  27. Litchfield J.T., Wilcoxon F. A simplified method of evaluating dose-effect experiments. J. Pharmacol. Exp. Ther. 1949;96:99–113. [PubMed] [Google Scholar]
  28. Loose L.D. Macrophage induction of T-suppressor cells in pesticide-exposed and protozoan-infected mice. Environ. Health Perspect. 1982;43:89–97. doi: 10.1289/ehp.824389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Loose L.D., Silkworth J.B., Charbonneau F., Blumenstock F. Environmental chemical-induced macrophage dysfunction. Environ. Health Perspect. 1981;39:79–91. doi: 10.1289/ehp.813979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Marsh M., Bolzau E., Helenius A. Penetration of Semliki Forest virus from acidic prelysosomal vacuoles. Cell. 1983;32:931–940. doi: 10.1016/0092-8674(83)90078-8. [DOI] [PubMed] [Google Scholar]
  31. Nagarkatti P.S., Sweeney G.D., Gauldie J., Clark D.A. Sensitivity to suppression of cytotoxic T cell generation by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is dependent on the Ah genotype of the murine host. Toxicol. Appl. Pharmacol. 1984;72:169–176. doi: 10.1016/0041-008x(84)90261-8. [DOI] [PubMed] [Google Scholar]
  32. Omann G.M., Lakowicz J.R. Interaction of chlorinated hydrocarbon insecticides with membranes. Biochim. Biophys. Acta. 1982;684:83–95. doi: 10.1016/0005-2736(82)90052-9. [DOI] [PubMed] [Google Scholar]
  33. Poland A., Glover E. 2,3,7,8-Tetrachlorodibenzo-p-dioxin: Segregation of toxicity with the Ah locus. Mol. Pharmacol. 1980;17:86–94. [PubMed] [Google Scholar]
  34. Prevost C.Le, Virelizier J.L., Dupuy J.M. Immunopathology of mouse hepatitis virus type 3 infection. III. Clinical and virologic observation of a persistent viral infection. J. Immunol. 1975;115:640–643. [PubMed] [Google Scholar]
  35. Quigley J.P., Rifkin D.B., Reich E. Lipid studies of Rous Sarcoma virus and host-cell membranes. Virology. 1972;50:550–564. doi: 10.1016/0042-6822(72)90406-0. [DOI] [PubMed] [Google Scholar]
  36. Siddel S., Wege H., Ter Meulen V. The structure and replication of coronaviruses. Curr. Top. Microbiol. Immunol. 1981;99:131–163. doi: 10.1007/978-3-642-68528-6_4. [DOI] [PubMed] [Google Scholar]
  37. Tamura T., Kai C., Sakaguchi A., Ishida T., Fujiwara K. The role of macrophages in the early resistance to mouse hepatitis virus infection in nude mouse. Microbiol. Immunol. 1979;23:965–974. doi: 10.1111/j.1348-0421.1979.tb00527.x. [DOI] [PubMed] [Google Scholar]

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