Skip to main content
NCBI home page
Elsevier - PMC COVID-19 Collection logoLink to Elsevier - PMC COVID-19 Collection
. 2002 Nov 12;8(1):9–18. doi: 10.1016/0166-0934(84)90036-3

A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes

JA Cowley 1, GA Tannock 1, Richard D Barry 1
PMCID: PMC7173268  PMID: 6323512

Abstract

A simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses utilizing adsorption to erythrocytes is described. The method involves virus growth in the presence of [35S]methionine, adsorption of clarified culture medium with a 0.5% suspension of either guinea-pig or chicken erythrocytes and analysis of the virus-erythrocyte aggregates by sodium dodecyl sulphate-polya-crylamide gel electrophoresis (SDS-PAGE). All of the structural proteins can be detected using this procedure, and the protein profiles of virus-adsorbed erythrocyte complexes compare extremely well with those of sucrose density gradient purified virus preparations.

Keywords: influenza virus, parainfluenza virus, erythrocyte adsorption, structural proteins

References

  1. Ada G.L., Perry B.T. J. Gen. Microbiol. 1956;14:623. doi: 10.1099/00221287-14-3-623. [DOI] [PubMed] [Google Scholar]
  2. Andrewes C., Pereira H.G., Wildy P. Viruses of Vertebrates. 4th ed. Bailliere Tindall; London: 1978. [Google Scholar]
  3. Chamberlain J.P. Anal. Biochem. 1979;98:132. doi: 10.1016/0003-2697(79)90716-4. [DOI] [PubMed] [Google Scholar]
  4. Choppin P.W., Compans R.W. In: Fraenkel-Conrat H., Wagner R.R., editors. Vol. 4. Plenum Press; New York: 1975. p. 95. (Comprehensive Virology). [Google Scholar]
  5. Cowley J.A., Barry R.D. J. Gen. Virol. 1983;64:2117. doi: 10.1099/0022-1317-64-10-2117. [DOI] [PubMed] [Google Scholar]
  6. Goswami K.K.A., Russell W.C. J. Gen. Virol. 1982;60:177. doi: 10.1099/0022-1317-60-1-177. [DOI] [PubMed] [Google Scholar]
  7. Gottschalk A. In: Burnet F.M., Stanley W.M., editors. Vol. 3. Academic Press; New York: 1959. p. 51. (The Viruses). [Google Scholar]
  8. Guskey L.E., Bergtrom G. J. Gen. Virol. 1981;54:115. doi: 10.1099/0022-1317-54-1-115. [DOI] [PubMed] [Google Scholar]
  9. Hirst G.K. Science. 1941;94:22. doi: 10.1126/science.94.2427.22. [DOI] [PubMed] [Google Scholar]
  10. Hoyle L., Home R.W., Waterson A.P. Virology. 1961;13:448. doi: 10.1016/0042-6822(61)90276-8. [DOI] [PubMed] [Google Scholar]
  11. Kingsbury D.W. In: Molecular Biology of Animal Viruses. Nayak D.P., editor. Marcel Decker; New-York: 1977. p. 349. [Google Scholar]
  12. Klenk H.-D., Scholtissek C., Rott R. Virology. 1972;49:723. doi: 10.1016/0042-6822(72)90529-6. [DOI] [PubMed] [Google Scholar]
  13. Laemmli U.K. Nature (London) 1970;227:680. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Lamb R.A., Choppin P.W. Virology. 1976;74:504. doi: 10.1016/0042-6822(76)90356-1. [DOI] [PubMed] [Google Scholar]
  15. Laver W.G. Adv. Virus Res. 1973;18:57. doi: 10.1016/s0065-3527(08)60262-6. [DOI] [PubMed] [Google Scholar]
  16. Lazarowitz S.G., Compans R.W., Choppin P.W. Virology. 1971;46:830. doi: 10.1016/0042-6822(71)90084-5. [DOI] [PubMed] [Google Scholar]
  17. Lazarowitz S.G., Compans R.W., Choppin P.W. Virology. 1973;52:199. doi: 10.1016/0042-6822(73)90409-1. [DOI] [PubMed] [Google Scholar]
  18. McClelland L., Hare R. Can.J. Publ. Hlth. 1941;32:530. [Google Scholar]
  19. Palmer D.F., Dowdle W.R., Coleman M.T., Schild G.C. Advanced Laboratory Techniques for Influenza Diagnosis. U.S. Department of Health, Education and Welfare; 1975. p. 32. (Immunology Series No. 6. Procedural Guide). [Google Scholar]
  20. Scheid A., Choppin P.W. J. Virol. 1973;11:263. doi: 10.1128/jvi.11.2.263-271.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Scheid A., Caliguiri L.A., Compans R.W., Choppin P.W. Virology. 1972;50:640. doi: 10.1016/0042-6822(72)90418-7. [DOI] [PubMed] [Google Scholar]
  22. Schulze I.T. Vol. 18. Academic Press; New York: 1973. p. 1. (Advances in Virus Research). [Google Scholar]
  23. Shimokata K., Ito Y., Nishiyama Y., Kimura Y. J. Gen. Virol. 1980;48:407. doi: 10.1099/0022-1317-48-2-407. [DOI] [PubMed] [Google Scholar]
  24. Skehel J.J. Virology. 1972;49:23. doi: 10.1016/s0042-6822(72)80004-7. [DOI] [PubMed] [Google Scholar]
  25. Stanley P., Haslam E.A. Virology. 1971;46:764. doi: 10.1016/0042-6822(71)90078-x. [DOI] [PubMed] [Google Scholar]
  26. White D.O. Curr. Trop. Microbiol. Immunol. 1974;63:1. [Google Scholar]

Articles from Journal of Virological Methods are provided here courtesy of Elsevier

RESOURCES