Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Hcrt-tTA | Tabuchi et al., 2014 | RRID:IMSR_APB:7778 | |
| Genetic reagent (Mus musculus) | Pmch-tTA | Tsunematsu et al., 2014 | RRID:IMSR_RBRC05844 | |
| Genetic reagent (Mus musculus) | TetO DTA | Jackson lab | RRID:IMSR_JAX:008468 | |
| Genetic reagent (Mus musculus) | Hcrt-tTA;TetO DTA | Tabuchi et al., 2014 | RRID:MGI:5583048 | |
| Genetic reagent (Mus musculus) | Hcrt-tTA; Pmch-tTA; TetO DTA | This paper | Mating of the Hcrt-tTA; TetO DTA with the Pmch-tTA mice | |
| Antibody | Rabbit anti-Pmch antibody | Sigma-Aldrich | RRID:AB_260690 | (1:1000) |
| Antibody | Goat polyclonal anti-Hcrt | Santa Cruz Biotechnology | RRID:AB_653610 | (1:1000) |
| Antibody | Mouse anti-tryptophan hydroxylase antibody | Sigma-Aldrich | RRID:AB_261587 | (1:500) |
| Antibody | Rabbit anti-tyrosine hydroxylase antibody | Merck | RRID:AB_390204 | (1:1000) |
| Antibody | Biotinylated horse anti-goat IgG antibody | Vector Laboratories | RRID:AB_2336123 | (1:1000) |
| Antibody | Biotinylated goat anti-rabbit/mouse IgG antibody | Vector Laboratories | RRID:AB_2313606 | (1:1000) |
| Sequence-based reagent | Hcrt | ACD Bio | Cat No. 490461-C2; RRID:SCR_012481 | (1:1500) |
| Sequence-based reagent | Pmch | ACD Bio | Cat No. 478721-C1; RRID:SCR_012481 | (1:1500) |
| Sequence-based reagent | Slc32a1 (vGAT) | ACD Bio | Cat No. 319191-C4; RRID:SCR_012481 | (1:750) |
| Sequence-based reagent | Slc17a6 (vGlut2) | ACD Bio | Cat No. 319171-C3; RRID:SCR_012481 | (1:750) |
| Chemical compound, drug | Opal520 | PerkinElmer | RRID:SCR_012163 | (1:1000) |
| Chemical compound, drug | Opal620 | PerkinElmer | RRID:SCR_012163 | (1:1000) |
| Chemical compound, drug | Chocolate | Meiji | Milk chocolate | (1.7–1.9 g/once) |
| Chemical compound, drug | Clomipramine | Sigma-Aldrich | C7291-1G; RRID:SCR_008988 | (15 mg/kg) |
| Chemical compound, drug | Modafinil | Cephalon Inc (Teva Pharmaceutical Industries Ltd.) | (100 mg/kg) | |
| Software, algorithm | Origin 2017 | Lightstone | RRID:SCR_014212; version 2017 | |
| Software, algorithm | SleepSign | Kissei Comtec | ||
| Software, algorithm | ImageJ | https://imagej.nih.gov/ij/ | RRID:SCR_002285 |