Figure 4. NP2631 ∩ dsx^FLP neurons in fruF males promote courtship, but not aggressive, behavior.

(A, B) Boxplots of time orienting (A₁, B₁), lunges (A₂, B₂), and wing extension (A₃, B₃) by the tester flies during the time windows 1–4 (see Figure 1D). Testers’ genotypes and pair numbers are indicated below the plots. Gray lines represent single testers. Target flies are either group-housed wild-type males (A) or mated females (B). In gray: **p<0.01, n.s. p>0.05 (Kruskal-Wallis one-way ANOVA and post-hoc Wilcoxon signed rank test). In black: **p<0.01, n.s. p>0.05 (Kruskal-Wallis one-way ANOVA and post-hoc Mann-Whitney U-test). (C) Comparison of wing extension performed by flies that express CsChrimson:tdTomato under the control of NP2631 and dsx^FLP in males (data from Figure 3—figure supplement 1I) or in fruF males (data from A, (B), during the time window 4. Sex of tester and target flies is indicated above the panels. Number of pairs tested is indicated below the panels. **p<0.01, n.s. p>0.05 (Mann-Whitney U-test). (D) Models of the function of NP2631 ∩ dsx^FLP neurons in the context of dsx and fru.

Figure 4—figure supplement 1. Rasters of behaviors induced by the optogenetic activation of NP2631 ∩ dsx^FLP neurons in fruF males.

(A, B) Rasters of time orienting (A₁, B₁), lunges (A₂, B₂), and wing extensions (A₃, B₃) performed by fruF male tester flies that express CsChrimson:tdTomato under the control of NP2631 and dsx^FLP (the same datasets as used in Figure 4A, (B). Pink bar: LED-on periods, horizontal bar: 10 min, vertical bar: 10 flies. LED stimulation condition is indicated at the bottom.