Figure 9. Identification and characterization of glial cell sub-types.

(A) t-SNE plot highlighting glial cell clusters. Clusters 23, 24, 70, 98, and 106 are shown in black, as they were determined to be glia based on differential expression of established glial and neuronal biomarkers (Figure 1—figure supplement 7). (B) t-SNE plot showing sub-clustering analysis of glial cells. Distinct clusters are color-coded and named. (C) Expression of established glial subtype-specific biomarkers used to determine cluster identity. astrocytic leucine-rich repeat molecule (alrm), for astrocytes; I’m not dead yet (Indy) for surface glia; wrapper for cortex glia; Excitatory amino acid transporter 2 (Eaat2) for ensheathing glia. Expression shown in black, intensity is proportional to the log-normalized expression levels. (D) Dot plot of the top 10 genes in each glial sub-type based on fold-enrichment (Figure 9—source data 1). Size of dots represent percent of cells in cluster expressing gene of interest (GOI); intensity of color reflects average scaled expression. Figure 9—source data 1 contain the full list of significantly enriched genes.

Figure 9—figure supplement 1. Identification of neuronal and glia clusters.

(A) t-SNE plot showing distribution of each replicate (Rep1-4) within the glial sub-clustering. (B) Heatmap showing Pearson correlations coefficients (−1 to +1) between each replicate per glial sub-cluster. Note replicate 1 does not contribute to the astrocyte-like or cortex glial clusters (grey). Replicates 2, 3, and 4 contributed to all clusters. Replicate 1 did not have any constituents in astrocyte-like or cortex glia. Replicate 1 was the smallest replicate in the whole data set and only contributed 13 of the 232 cells depicted in the glial sub-clustering.