FIG. 5.

Analysis of protein patterns. (a) Fluorescence micrographs and (b) corresponding mean fluorescence intensity values of protein patterns in PDMS and 3D-printed chips. Protein-G and BSA patterns are labeled with fluorescent IgG antibody. Before labeling in the PDMS chip, bare Protein-G pattern is analyzed. PDMS chip is also used to analyze the BSA pattern. Protein patterns of the PDMS chip are inspected after unclamping the PDMS channel from the glass slides. Protein patterns and channels are in between white and red dashed lines, respectively. Scale bars are 200 μm. Data are statistically analyzed using one-way ANOVA combined with the Newman–Keuls multiple comparisons test. Fluorescence intensities in different cases are compared with PDMS chips, and (****) indicates p < 0.0001. (c) Log plot of IgG concentration and the corresponding fluorescent signal on the Protein-G pattern inside the 3D-printed microfluidic chip. Linear fit to the data with a coefficient of determination (r²) is shown in the figure.