Skip to main content
. 2020 Mar 3;10(3):392. doi: 10.3390/biom10030392

Figure 2.

Figure 2

Zn (II)-curc does not affect ER stress in wtp53-carrying cells. (a) (left panels) Western blot analysis of BiP and p53 protein levels in RKO and HCT116 cells treated with Zn(II)-curc (100 µg/mL) for 24 h. Densitometric analysis (right panels) was performed using Image J software to calculate the ratio of BiP and p53 protein levels vs. β-actin. Histograms represent the mean ± SD of three independent experiments. * p ≤ 0.05. (b) Western blot analysis of BiP protein levels in RKO and HCT116 cells treated with Tunicamycin (Tn) (1 µg/mL) for 4 h. (upper panel) Densitometric analysis was performed using Image J software to calculate the ratio of BiP protein levels vs. β-actin. Histograms represent the mean ± SD of three independent experiments. * p ≤ 0.05. (c) Total mRNA was extracted from RKO and HCT116 cells treated with Tunicamycin (Tn) for 24 h, and Xbp1 gene expression was assayed by the PCR of reverse-transcribed cDNA. Densitometric analysis was performed using Image J software to calculate the Xbp1s/28S ratio. Histograms represent the mean ± SD of three independent experiments. * p ≤ 0.05.