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. 2020 Feb 24;9(2):152. doi: 10.3390/pathogens9020152

Figure 3.

Figure 3

IgG antibody isotypes (IgG1, IgG2a, and IgG2b) elicited after immunization with rL7/L12, rOmp25, and divalent vaccine (Omp25+L7/L12): The recombinant B. abortus proteins rL7/L12, rOmp25, and Omp25+ L7/L12 were immunized in mice and isolation of serum samples was done from tail veins on day 42. Estimation of IgG isotype levels in serum of immunized mice was done through ELISA. The antibodies used for ELISA were horseradish peroxidase (HRP) conjugated anti-mouse IgG1, IgG2a, and IgG2b antibodies and data is plotted as (mean (OD450 ± SD)).