Figure 4.
Bactericidal activity of SMAP-modified LysECD7 against the A. baumannii Ts 50-16 strain over the pH gradient range at a protein concentration of 0.5 µg/mL. The bacterial suspension was diluted in 20 mM Tris HCl buffer with different pH values (5.0 to 9.0) to 1 to 3 × 105 CFU/mL and mixed with the proteins. For all experiments, the mean values are shown from three independent experiments ± SD. An asterisk (*) indicates a significant difference in bactericidal activity compared to the LysECD7 enzyme (p < 0.05, two-way ANOVA with Dunnett’s multiple comparisons test).