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. 2020 Mar 18;10(3):467. doi: 10.3390/biom10030467

Figure 1.

Figure 1

Analysis of annexin 2 (ANXA2) expression in PC3 and bone progenitors’ cells grown in co-culture conditions. Effect of heme oxygenase 1 (HO-1) induction. (A) Schematic representation of the co-culture transwell system of PC3 and Raw264.7 cells. Expression levels of (B,C) HO-1 assessed by real-time PCR (RT-qPCR) and WB; (D) ANXA2 and (E) ANXA2-R assessed by RT-qPCR in PC3 cells pre-treated or not with hemin (50 μM; 24 h), grown alone or in co-culture with Raw264.7 cells. The values were relativized using PPIA as a reference gene and normalized to controls. Expression levels of Anxa2 assessed by RT-qPCR (F) (relativized to 36b4 as a reference gene) or Western blot (G) (relativized to β-actin, as a reference protein), in Raw264.7 cells grown alone or in co-culture with PC3 cells pre-treated or not with hemin (50 μM; 24 h). (H) Expression levels for ANXA2 in primary tumor vs. different sites of metastasis (bone, liver, lymph node, lung), analyzed on the GSE74685 dataset (n = 171). Significant differences: * p < 0.05; ** p < 0.01; *** p < 0.001.