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. 2019 Apr 9;40(11):1415–1426. doi: 10.1093/carcin/bgz070

Figure 5.

Figure 5.

β-hCG induces HR in BRCA1 mutant but not in wild-type cells. (A) HR assay showing the level of recombination in HCC1937 and HCC1937/wt BRCA1 upon supplementation of β-hCG. d1 and d2 plasmid were taken as internal control. The d1 and d2 were obtained as 546bp and 258bp products whereas recombination product (HR) was obtained at 420bp. (B) HR levels were normalized to HCC1937 and quantitation of HR was given on right panel. (C) Quantitative Real Time PCR (qRT-PCR) analysis showing the level of HR in HCC1937 and HCC1937/wt BRCA1 cells treated with 17AAG in the presence or absence of β-hCG. HR levels were normalized to HCC1937 + 17AAG. (D) qRT-PCR analysis showing the level of HR in HCC1937 and mutant BRCA1 stably silenced HCC1937 cells (HCC1937 sh BRCA1). (E) qRT-PCR analysis showing the level of HR in HCC1937 and mutant BRCA1 stably silenced HCC1937 cells (HCC1937 sh BRCA1) treated with 17AAG in the presence or absence of β-hCG. (F) Pictorial depiction of HR in BRCA1 mutant and wild-type cells with respect to β-hCG. Full blots of cropped images are included in supplementary information. **P < 0.01.