Figure 5.

Multiplex assay for cytokines secreted from retinal cells treated with Hcy and cbs^+/− mice brain. Multiplex cytokine assay analysis from conditioned media of HRECS and ARPE-19 cells in response to 50 µM and 100 µM Hcy, respectively. (A,B) Showed and confirmed the increased of the pro-inflammatory cytokines and downregulation of the anti-inflammatory cytokine (IL4), while brain cytokine array from the hippocampal area of the cbs^+/− mice brain (C) showed a significant decrease in IL13, MCP-1, and increase in Exotoxin and IL3. Three replicates per sample and two replicates per cytokine were performed (* p < 0.05, ** p < 0.01, *** p < 0.001). Data are expressed as mean ±SD.