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Flow cytometric analysis was performed to determine apoptotic activity in GNST-ITC-treated MCF-7 cells by Annexin-V/PI double staining. MCF-7 cells were treated for 24, 48, and 72 h: (A–D) control and 24 h, 48 h, and 72 h treated cells respectively. (E) Bar chart shows percentage of cells distribution after the treatment. Values are presented as means ± SD of triplicate experiments. Significant difference (p < 0.05) as compared to control is indicated by asterisk.
