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. 2020 Mar 9;25(5):1230. doi: 10.3390/molecules25051230

Figure 6.

Figure 6

Effects of melatonin and PD98059 on cell proliferation and INHA expression in chicken Sertoli cells. (A) The protein level of p-ERK1/2 (n = 3). (B) Quantitative analyses of the p-ERK protein results. (C) INHA mRNA levels of chicken Sertoli cells (n = 3). (D) INHA levels of chicken Sertoli cells by ELISA (n = 3). (E) Cell viability of chicken Sertoli cells (n = 3). (F) The EdU method was used to measure chicken Sertoli cell proliferation (×10 magnification; n = 3). (G) Statistical analysis of data in (F). The relative mRNA expression levels of (H) proliferating cell nuclear antigen (PCNA) and (I) Cyclin D1 (CCND1; n = 3 for both). (J) The relative protein expression levels of CCND1 and PCNA. Quantitative analyses of the (K) CCND1 and (L) PCNA protein results (n = 3 for both). *** p < 0.001, ** p < 0.01 or * p < 0.05.