Decay in neutralizing antibodies was evaluated at 0, 6, 12, 24 and 36 months for Ad4, Ad5, and Ad7 in the pre-ART cohort (A). Only the 12 month time point was analyzed for the pre-ART (A) for Ad26, Ad28, Ad45 and Ad48 and all Ads for the post-ART cohort (B) due to sampling limitations. Significant change in average neutralization was evaluated as a comparison to 0 months or enrollment (ENR) by Mann-Whitney analysis. (* P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001)