Skip to main content
. 2020 Apr 9;9:e55370. doi: 10.7554/eLife.55370

Figure 7. Cooling activates mouse but not squirrel CNGA3 in HEK293T cells.

(A–C) Exemplar whole-cell currents (left) and quantification of current amplitude at 90 mV (middle) and −90 mV (right) recorded in HEK293T cells expressing empty vector (control) or CNGA3 in response to a voltage ramp in the presence of 2 µM intracellular cGMP, at indicated temperatures. Currents were elicited by voltage ramps from −100 mV to 100 mV from a holding potential of −60 mV. (D) Quantification of the magnitude of cold activation of CNGA3 current in HEK293T cells in the presence of 2 µM intracellular cGMP at 90 mV (left) and −90 mV (right). (E) Exemplar whole-cell current traces evoked in HEK293T cells expressing mCNGA3, sqCNGA3 or empty vector (control) by voltage ramps from −100 mV to 100 mV from a holding potential of −60 mV in the presence of saturating 100 µM intracellular cGMP at 22°C. (F) Quantification of current amplitude at 90 mV (left) and −90 mV (right) in the presence of saturating 100 µM intracellular cGMP at 22°C. Data are mean ± SEM. **p<0.01; ***p<0.001, paired t-test (A–C) or Dunnett’s test (D, F). Each dot represents individual cell.

Figure 7—source data 1. Reversible activation of mouse CNGA3 in HEK293T cells.

Figure 7.

Figure 7—figure supplement 1. Reversible activation of mouse CNGA3 in HEK293T cells.

Figure 7—figure supplement 1.

Exemplar whole-cell current traces obtained from mCNGA3 in the presence of 2 µM intracellular cGMP in response to temperature ramps. Currents were elicited by voltage ramps from −100 mV to 100 mV from a holding potential of −60 mV.