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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Methods. 2019 Oct 25;177:50–57. doi: 10.1016/j.ymeth.2019.10.010

Figure 1. Schematic Presentation for the Isolation Process of MSC-Derived MVs.

Figure 1.

Condition media harvested from MSCs is first centrifuged at 300 ×g for 10 minutes. Next, the supernatant is ultracentrifuged at 16,500 ×g for 20 minutes following by a washing step. Finally, the MVs pellet is washed and resuspended in PBS and kept at −80 °C.