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. 2020 Apr 22;30:105568. doi: 10.1016/j.dib.2020.105568

Data on synthesis and characterization of new p-nitro stilbene Schiff bases derivatives as an electrochemical DNA potential spacer

Nur Zafirah Mohd Izham a, Hanis Mohd Yusoff a,b,, Irshad Ul Haq Bhat a,b, Tomoaki Endo c, Hiroshi Fukumura c,d, Eunsang Kwon e, Shin-Ichiro Yoshida e, Asnuzilawati Asari a, Uwaisulqarni M Osman a,b, Mohd Sukeri Mohd Yusof a,b
PMCID: PMC7184124  PMID: 32368595

Abstract

The structural investigation of synthesized compounds can be carried out by various spectroscopic techniques. It is an important prospect in order to elucidate the structure of the desired products before being further utilized. The preparation of new p-nitro stilbene Schiff base derivatives as an electrochemical DNA potential spacer was synthesized using (E)-4-(4-nitrostyryl)aniline from Heck reaction with aldehydes in ethanolic solution. The data presented here in this article contains FTIR, UV-Vis and 1H and 13C NMR of (E)-4-(4-nitrostyryl)aniline and nitrostyryl aniline derivatives.

Keywords: Aldehyde, Imine, Heck reaction, Schiff base derivatives, Stilbene, Spacer


Specifications table

Subject Chemistry
Specific subject area Spectroscopy
Type of data Table
Figure
Scheme
How data were acquired FTIR (Shimadzu IRTracer-100 Fourier Transform Infrared Spectrometer) ranges from 400 to 4000 cm−1 by using single reflection ATR;
UV-Vis (double beam Shimadzu UV-1800 spectrophotometer) ranges from 190 to 800nm by using acetonitrile as solvent in 1cm3 cuvette;
1H and 13C nuclear magnetic resonance (NMR) spectra (Bruker Avance II 400 spectrophotometer) using deuterated dimethylsulfoxide (DMSO-d6) as solvent.
Data format Raw and analysed
Parameters for data collection All compounds were synthesized at room temperature.
Description of data collection The synthesized compounds from Heck and Schiff base reactions were characterized by spectroscopic method for establishing the structure of compounds.
Data source location Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
Data accessibility http://dx.doi.org/10.17632/fw86ngty6c.1

Value of the data

  • The following data shows new derivatives synthesized from (E)-4-(4-nitrostyryl)aniline, which are important in structure elucidation and are useful to researchers who are developing spacer to be applied on substrate used in electrochemical DNA sensor.

  • For future investigations, the details in these data could be used for comparison study with previous spacers, which in turn could help in further understanding of the significant role of substituent attached at the (nitrostyryl)aniline terminal end.

  • The details in this data could be extended towards other field such as for antibacterial study and other biological application as many studies has reported that imine structure could inhibit the bacterial growth.

1. Data

Previously, Hassan et al. (2018) had synthesized several stilbene Schiff base derivatives with various alkyl chain lengths [1], [2], [3]. This paper deals about the identification and characterization of Heck product and three (nitrostyryl)aniline derivatives of the same length but with different terminal end as a comparison with alkyl terminal end. This paper deals about the identification and characterization of Heck product and three (nitrostyryl)aniline derivatives. It describes the preparation of samples prior the spectroscopy measurements which presented accordingly. Scheme 1 describes the overall reaction involved in producing (E)-N-(4-ethylbenzylidene)-4-((E)-4-nitrostyryl)aniline (NO2Et), (E)-N-(4-methylbenzylidene)-4-((E)-4-nitrostyryl)aniline (NO2Me) and (E)-N-(4-methoxybenzylidene)-4-((E)-4-nitrostyryl)aniline (NO2OMe), which includes Heck and Schiff base reactions. Table 1 and Fig. 1 describe the comparative assessment of FTIR of all the samples. Whereas, Table 2 and Fig. 2 describe the UV-vis data comparison of four products produced. Tables 3 and 4 tabulate the 1H and 13C NMR of (E)-4-(4-nitrostyryl)aniline (Heck 1) and three imine products. Figs. 4, 6, 8 and 10 display the images of NMR spectra of Figs. 3, 5, 7 and 9 that are being studied, respectively.

Scheme 1.

Scheme 1

Overall reaction scheme.

Table 1.

Frequencies of selected bands of diagnostic importance from the IR spectra of Heck 1 and nitrostyryl aniline Schiff bases derivatives.

Compound frequency (cm−1)
Assignment
Heck 1 NO2Et NO2Me NO2OMe
3483 & 3383 - - - NH2
1496 & 1307 1458 & 1334 1419 & 1338 1458 & 1338 N=O
3070-2900 3074-2877 3097-2868 3074-2843 C-H stretching
- 1458 - - CH2 bending
- 1373 1377 1373 CH3 bending
- 1681 1681 1681 C=N
- - - 1253 & 1022 C-O stretching
1581 1516 1516 1508 C=C alkene

Fig. 1.

Fig 1

Infrared spectra of compounds formed. (A) Heck 1, (B) NO2Et, (C) NO2Me and (D) NO2OMe.

Table 2.

Wavelengths of UV-visible spectra values of Heck 1 and nitrostyryl aniline Schiff bases derivatives.

Peak Compound wavelength, nm (absorbance)
Assignment
Heck 1 NO2Et NO2Me NO2OMe
1 408.0 (0.42) 381.50 (0.399) 382.5 (0.413) 384.5 (0.242) R-NO2 and C=N (n → π*)
2 288.0 (0.27) 276.50 (0.355) 277.0 (0.389) 276.5 (0.306) C=C aromatic (π → π*)

Fig. 2.

Fig 2

UV-Visible spectra of Heck 1, NO2Et, NO2Me and NO2OMe in acetonitrile, respectively.

Table 3.

Chemical shifts of 1H NMR values of Heck 1 and nitrostyryl aniline Schiff bases derivatives.

Signal 1H NMR chemical shifts of compounds (ppm)
Assignment
Heck 1 NO2Et NO2Me NO2OMe
2 8.17, 8.19 (d) 8.15, 8.17 (d) 8.24, 8.26 (d) 8.23, 8.26 (d) Aromatic
3 7.72, 7.75 (d) 7.82, 7.84 (d) 7.87, 7.89 (d) 7.90, 7.92 (d) Aromatic
5 7.03, 7.07 (d) 7.01, 7.05 (d) 7.56, 7.60 (d) 7.55, 7.59 (d) C=C
6 7.34 (s) 6.58, 6.61 (d) 7.41, 7.45 (d) 7.40, 7.44 (d) C=C
8 7.36, 7.38 (d) 7.72, 7.74 (d) 7.84, 7.86 (d) 7.89, 7.87 (d) Aromatic
9 6.59, 6.61 (d) 7.33 (s) 7.32 (s) 7.30, 7.32 (d) Aromatic
11 5.57 (s) 9.95 (s) 8.64 (s) 8.60 (s) NH2
13 - 7.36, 7.38 (d) 7.41, 7.45 (d) 7.72, 7.74 (d) Aromatic
14 - 7.43, 7.45 (d) 7.34, 7.36 (d) 7.08, 7.10 (d) Aromatic
16 - 2.68, 2.70 (d) 2.39 (s) 3.85 (s) Alkane
17 - 1.20 (s) - - Alkane

Table 4.

Chemical shifts of 13C NMR values of Heck 1 and nitrostyryl aniline Schiff bases derivatives.

Signal 13C NMR chemical shifts of compounds (ppm)
Assignment
Heck 1 NO2Et NO2Me NO2OMe
1 150.48 161.01 146.55 146.50 Aromatic
2 124.51 120.83 124.54 124.54 Aromatic
3 129.18 129.18 129.27 129.35 Aromatic
4 145.83 150.40 144.67 144.69 Aromatic
5 & 6 126.67 124.51 127.68 127.65 C=C
7 120.80 145.80 134.54 134.26 Aromatic
8 134.84 130.19 129.95 131.08 Aromatic
9 114.26 114.31 122.13 122.08 Aromatic
10 207.00 193.24 152.13 152.34 Aromatic
11 - 207.38 161.02 160.43 Imine
12 - 134.81 133.94 128.63 Aromatic
13 - 129.06 133.31 133.35 Aromatic
14 - 126.69 126.26 114.79 Aromatic
15 - 151.80 142.19 162.51 Aromatic
16 - 28.84 21.67 55.90 Alkane
17 - 15.79 - - Alkane

Fig. 4.

Fig 4

(A) 1H and (B) 13C NMR of Heck 1.

Fig. 6.

Fig 6

(A) 1H and (B) 13C NMR of NO2Et.

Fig. 8.

Fig 8

(A) 1H and (B) 13C NMR of NO2Me.

Fig. 10.

Fig 10

(A) 1H and (B) 13C NMR of NO2OMe.

Fig. 3.

Fig 3

Heck 1.

Fig. 5.

Fig 5

NO2Et.

Fig. 7.

Fig 7

NO2Me.

Fig. 9.

Fig 9

NO2Ome.

2. Experimental Design, Materials, and Methods

2.1. Materials

4-vinylaniline, 4-ethylbenzaldehyde, p-tolualdehyde and p-anisaldehyde (98%) were purchased from Acros Organics; N,N-dimethylformamide and dichloromethane were from Fisher Scientific, while 1-iodo-4-nitrobenze (98%), trimethylamine and ethanol absolute were purchased from Sigma-Aldrich, R&M Chemicals and HmbG Chemicals, respectively. All chemicals were used as received.

2.2. Instrumentation

The structure of synthesized products was established by spectral data obtained by different spectroscopic instruments. FTIR, UV-Vis and NMR were recorded by Shimadzu IRTracer-100 Fourier Transform Infrared Spectrometer, double beam Shimadzu UV-1800 spectrophotometer and Bruker Avance II 400 spectrophotometer, respectively. Reaction progresses of the compounds were monitored by Thin layer chromatography (TLC) using silica gel 60 F254, 0.25 mm thick plastic plates [1,3].

2.3. Synthesis of (E)-4-(4-nitrostyryl)aniline

A substituted aryl halide (1 mol) was added to a mixture of N, N-dimethylformamide (3 ml) and 4-vinylaniline (1 mol, 0.4 g), trimethylamine (3 ml) as base and bis(triphenylphosphine)palladium chloride (40 mg) as catalyst in a three-neck round bottom flask. The resulting mixture was stirred well and refluxed for 24 hours at 70-80°C. The completion of reaction was monitored by TLC. The reaction mixture was filtered and thoruoghly washed by dichloromethane. Next, the solvent was evaporated by the rotary evaporator and run for column chromatography in order to obtain the the product of (E)-4-(4-nitrostyryl)aniline (Heck 1) (Fig. 3) as previously produced by [3].

2.4. Synthesis of Schiff bases derivatives

Schiff base method of [4] was modified to produce imine compounds. In a Dean-stark flask 1 mol of the synthesized product from Heck reaction dissolved in ethanol (50 ml), 0.1 ml of commercial aldehyde (p-tolualdehyde or p-ansaldehyde) in ethanol (10 ml) was added at 60°C. The reaction mixture was refluxed for 2 hours at 80°C. The reaction mixture was cooled down and the product was precipitated. The yielded product was filtered off and recrystallized from hot acetonitrile [5]. Nitrostyryl aniline derivatives (Figs. 2, 7, 9) produced were weighed and the percentage yield was recorded.

2.5. Complete characterization description of the four compounds

(E)-4-(4-nitrostyryl)aniline (Heck 1): Brick-red powder; ATR: 3483 & 3388 (NH2, stretching), 1496 & 1307 (N=O), 3070-2900 (C-H stretching,)1581 (C=C alkene); UV–Vis spectrum [ACN, λmax nm (log ɛ, L mol−1 cm−1)]: 408.00 (4.62), 288.00 (4.43); 1H NMR (400MHz, DMSO-d6): δ 8.19-6.59 (d, 10H), 5.57 (s, 2H); 13C NMR (100MHz, DMSO-d6): δ 207.0, 150.48, 145.83, 129.18, 126.67, 124.51, 120.80, 114.26.

(E)-N-(4-ethylbenzylidene)-4-((E)-4-nitrostyryl)aniline (NO2Et): Yellow flakes; ATR: 1458 & 1334 (N=O), 3074-2877 (C-H, stretching), 1458 (CH2, bending), 1373 (CH3, bending), 1681 (C=N), 1516 (C=C alkene): UV–Vis spectrum [ACN, λmax nm (log ɛ, L mol−1 cm−1)]: 381.50 (4.60), 276.50 (5.55); 1H NMR (400MHz, DMSO-d6): δ 9.95 (s, 1H), 8.17-6.58 (d, 14H), 2.70-2.68 (d, 2H), 1.20 (s, 3H);); 13C NMR (100MHz, DMSO-d6): δ 207.38, 193.24, 161.01, 151.80, 150.40, 145.80, 134.81, 130.19, 129.18, 126.69, 129.06, 124.51, 120.83, 114.31, 28.84, 15.79.

(E)-N-(4-methylbenzylidene)-4-((E)-4-nitrostyryl)aniline (NO2Me): Yellow flakes; ATR: 1419 & 1338 (N=O), 3097-2868 (C-H, stretching), 1377 (CH3, bending), 1681 (C=N), 1516 (C=C alkene); UV–Vis spectrum [ACN, λmax nm (log ɛ, L mol−1 cm−1)]: 382.50(4.62), 277.00 (4.59); 1H NMR (400MHz, DMSO-d6): δ 8.64 (s, 1H), 8.26-7.32 (d, 14H), 2.39 (s, 3H); 13C NMR (100MHz, DMSO-d6): δ 161.02, 152.13, 146.55, 144.67, 142.19, 134.54, 133.94, 133.31, 129.95, 129.97, 127.68, 126.26, 124.54, 122.13, 21.67.

(E)-N-(4-methoxybenzylidene)-4-((E)-4-nitrostyryl)aniline (NO2OMe): Yellow flakes; ATR: 1458 & 1338 (N=O), 3074-2843 (C-H, stretching), 1373 (CH3, bending), 1681 (C=N), 1253 & 1022 (C-O, stretching), 1508 (C=C alkene); UV–Vis spectrum [ACN, λmax nm (log ɛ, L mol−1 cm−1)]: 384.5 (4.38), 276.5 (4.49); 1H NMR (400MHz, DMSO-d6): δ 8.60 (s, 1H), 8.26-7.32 (d, 14H), 2.39 (s, 3H); 13C NMR (100MHz, DMSO-d6): δ 162.51, 160.42, 152.34, 146.50, 144.69, 134.26, 133.35, 131.08, 129.35, 128.63, 127.65, 124.54, 122.08, 14.79, 55.90.

Acknowledgments

Authors gratefully acknowledge Ministry of Higher Education, Malaysia, for their financial support through Fundamental research grant scheme (FRGS) (FRGS/1/2015/SG01/UMT/02/3). A sincere thanks to National Institute of Technology, Sendai College and also Giant Molecular Analysis, Tohoku University, Japan for their help in completing a part of this work.

Footnotes

Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.dib.2020.105568.

Appendix. Supplementary materials

mmc1.xml (472B, xml)

References

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Associated Data

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Supplementary Materials

mmc1.xml (472B, xml)

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