Table 2.
Contents of TC, FC and CE in human primary macrophage-derived foam cells measured by HPLC (μg/mg cell protein).
| Group | TC | FC | CE | CE/TC (%) |
|---|---|---|---|---|
| sh-NC | 436.22 ± 13.86 | 181.28 ± 11.35 | 255.58 ± 14.22 | 59.89 |
| sh-CDKN2B-AS1 | 286.59 ± 10.38a | 124.35 ± 11.54a | 162.35 ± 13.86a | 64.19 |
| oe-NC | 443.25 ± 13.45 | 178.21 ± 10.35 | 265.34 ± 14.35 | 58.67 |
| oe-CDKN2B-AS1 | 635.45 ± 18.33b | 228.65 ± 15.38b | 407.53 ± 17.56b | 56.59 |
Note:
a
p < 0.05 vs. the sh-NC group.
b
p < 0.05 vs. the oe-NC group. All the experiments were repeated 3 times independently, and the data among multiple groups were analyzed by one-way analysis of variance; oe-NC, cells transduced with LV5-GFP empty vector; oe-CDKN2B-AS1, cells transduced with LV5-GFP-CDKN2B-AS1; sh-NC, cells transduced with pSIH1-H1-copGFP-sh-NC; sh-CDKN2B-AS1, cells transduced with pSIH1-H1-copGFP-sh-CDKN2B-AS1; TC, total cholesterol; FC, free cholesterol; CE, cholesterol ester; HPLC, high-performance liquid chromatography; NC, negative control.