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CCD-1 composition, evaluated factors and their respective levels.
| Factor | Level |
||||
|---|---|---|---|---|---|
| -1.5 | -1 | Central Point | 1 | 1.5 | |
| Glucose USP (gL−1) | -0.05∗∗ | 3.3 | 6.65 | 10.0 | 13.35 |
| Isolated soy protein (gL−1) | 5.0 | 10.0 | 15.0 | 20.0 | 25.0 |
| Malt extract (gL−1) | 3.5 | 7.0 | 10.5 | 14.0 | 17.5 |
| (NH4)2SO4 (gL−1) | 1.3 | 2.6 | 3.9 | 5.2 | 6.5 |
| CuSO4 (gL−1)∗ | 0.16 | ||||
| Chloramphenicol (gL−1)∗ | 0.1 | ||||
These factors remained unchanged for all combinations.
This value was assumed as 0.0.
