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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: Bone. 2020 Feb 4;133:115262. doi: 10.1016/j.bone.2020.115262

Figure 2: expression of TNAP, and ectonucleotidases in metaphysis-containing bone fragments, hypertrophic chondrocytes and osteoblasts.

Figure 2:

A: enzymes known to be involved in ATP hydrolysis into adenosine, and their inhibitor. B: RT-qPCR analysis of nucleotidase-encoding mRNA levels in the metaphysis-containing femurs and tibias of 7-day-old wildtype mice. Mouse primary chondrocytes (C, n=4 independent experiments) and primary osteoblasts (D, n=3) were cultured as detailed in the Materials and methods section and mRNA levels were quantified by RT-qPCR. * indicates a statistical difference with p<0.05; ** a difference with p<0.01; *** a difference with p<0.001.