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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: Bone. 2020 Feb 4;133:115262. doi: 10.1016/j.bone.2020.115262

Figure 5: absence of anti-inflammatory effects of TNAP in MSCs.

Figure 5:

A: effect of a 7-day treatment with IL-1β on TNAP activity in MSCs left undifferentiated or differentiated into osteoblasts (n=6). To eliminate the inter-experimental variations, TNAP activity was set to 100% in untreated cells. B: effect of a 7-day treatment with IL-1β on TNAP activity in murine primary osteoblasts (n=3). C: quantification of nucleotidase mRNA levels in undifferentiated MSCs by RT-qPCR (n=8 independent experiments with MSCs from 4 different donors). D: in undifferentiated MSCs pre-treated for 4 days with 1 ng/mL of IL-1β, the hydrolysis of 10 μM of ATP into Pi was measured by the Malachite green assay in the presence or absence of 25 μM of MLS-0038949 and 100 μM of ARL-67156 (n=8 with MSCs from 4 different donors). * indicates a statistical difference with p<0.05; ** a difference with p<0.01.