Figure 3. WFA synergizes the antiproliferative activity of PAC and CisPt against A549 cells.

The potency and efficacy of PAC, CisPt, and WFA against A549 cells were compared using the median-effect equation. (A) Dose-response plots depicting PAC, CisPt and WFA dose-dependent inhibition of cell proliferation of A549 cells. (B) Median-effect plot indicated PAC had lowest IC₅₀ followed by WFA and CisPt the highest. Preliminary combinations of PAC and CisPt, PAC and WFA and CisPt and WFA were tested. PAC+WFA (C) displayed the greatest efficacy, followed by CisPt + WFA (D–F). Isobologram analysis (G–I) and combination index (CI) values (<1) indicated that all the combinations were highly synergistic against A549 cells. Dose response curves were generated for WFA, PAC and combinations using Calcusyn 2.0 (Biosoft). A fractional effect of 1 means 100% cell kill by the drug (s), and zero means no effect (J–L).