Figure 8. WFA alone inhibited the growth and proliferation of resistant PAC resistant NSCLC cells.
(A) MTT assay depicting the in vitro inhibition of cellular proliferation of TR A549 and TS A549 (B) WFA significantly inhibited the growth of TR A549 (2.5 × 106 cells/mouse) tumor xenografts in athymic nude mice. (C) RT PCR and Western blot analysis indicated dose-dependent inhibition of the expression MDR1 in TR A549 after incubation with WFA for 48 h. (D) Annexin V/PI assay indicated dose-dependent induction of apoptosis in TR A549 cells after incubation with WFA (0–2 μM) for 24 h. (E) Western blot analysis confirmed the induction of apoptosis as indicated by the increased cleavage of PARP and caspase 3, increased expression of Bax, p21, phosphor-H3 while inhibiting the expression of Bcl2 and cyclin E2.