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. 2005 Jun 17;37(6):386–390. doi: 10.1111/j.1745-7270.2005.00058.x

Dose‐dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference

Guo‐Feng CHENG 1, Jiao‐Jiao LIN 1, Yi SHI 2, You‐Xin JIN 2,✉, Zhi‐Qiang FU 1, Ya‐Mei JIN 1, Yuan‐Cong ZHOU 2, You‐Min CAI 1,✉
PMCID: PMC7185640  PMID: 15944753

Abstract

Abstract The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. This protein is widely distributed in the adult female worm after pairing. Reverse transcription‐polymerase chain reaction (RT‐PCR) and immunofluorescence were employed to analyze the relationship between the RNAi effect and dsRNA dosage in the parasites. The results revealed that the inhibition of SjGCP expression by siRNA is dose‐dependent. RT‐PCR analysis showed that the SjGCP transcript level was reduced by 75% when 100 nM dsRNA was applied.

Edited by: Wang‐Yi LIU

Keywords: RNA interference, gynecophoral canal protein gene, Schistosoma japonicum, dosage

This work was supported by the grants from the Shanghai Important Science and Technology Key Program of China (No. 03DZ19231), the Shanghai Program for Phosphor Plan of China (No. 04QMX1462) and the National Natural Science Foundation of China (No. 30270311)

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