Figure 10.

Loss of Tpm3.1 Leads to a Reduction in Myosin IIB Immunofluorescence

Cultured hippocampal neurons of conditional Tpm3 knockout mice (Tp9 line). Arrowheads indicate neurons expressing Cre-GFP after either viral transduction (top panel) or lipofection (bottom panel). We used anti-myosin IIB to compare the distribution of myosin IIB in neurons expressing Cre-GFP and neighboring control neurons. Cre-GFP expressing neurons showed a lower intensity of myosin IIB immunofluorescence (t test). Box borders represent the 25^th and 75^th percentiles, whiskers represent minimum and maximum values less than 1.5x the interquartile range lower or higher than the 25^th or 75^th percentiles, respectively (Tukey style). Neurons expressing Cre-GFP; transduced: n = 12, 3 independent experiment; transfected n = 12, 3 independent experiments. Control neurons: transduced: n = 22; transfected: n = 31. ∗ denotes statistical significance, two-sample t test. ∗: p < 0.05. Scale bar: 10 μm.