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. 2020 Apr 12;23(5):101053. doi: 10.1016/j.isci.2020.101053

Figure 5.

Figure 5

Tpm3 Conditional Knockout Neurons Show a Reduced Accumulation of Ankyrin G at the AIS

(A) Ankyrin G immunofluorescence for GFP- (wild-type, WT) and Cre GFP-expressing (Tpm3 KO) neurons. β3-Tubulin was used to label neurons. Arrowheads indicate transfected neurons; arrows indicate axons of transfected neuron. Scale bar: 5 μm.

(B) Average relative ankyrin G fluorescence intensity for each group (Mann-Whitney U test). Columns represent mean values. Error bars represent standard error of mean. GFP: n = 63, 3 independent experiments; Cre-GFP: n = 61, 3 independent experiments. ∗ denotes statistical significance. ∗∗∗: p < 0.001.

(C) AIS localization indices (ALI) for neurons infected with GFP or Cre-GFP. The ALI of Cre-GFP infected neurons (0.87 ± 0.02, mean ± SEM, n = 57, 3 independent experiments) was lower than that of GFP-infected controls (0.95 ± 0.002, mean ± SEM, n = 61, 3 independent experiment, p < 0.001, Mann-Whitney U test). Black circles represent mean values. Box borders represent the 25th and 75th percentiles, whiskers represent minimum and maximum values less than 1.5x the interquartile range lower or higher than the 25th or 75th percentiles, respectively (Tukey style).

See also Figures S6–S9.