Table 1. Genes located inside neocentromeres.
The chromosomal locations, sizes, and GC content (%) for the native centromere and cen∆ mutants are shown. For the neocentromeres, gene ID, predicted function, and the amount of CENP-A coverage are indicated.
| Chr coor (bp) | Size (kb) | Size compared to native centromere (%) |
GC% | Genes spanned by neocentromere |
Gene ID | % covered by Neocentromere |
Exons inside neocentromere |
|
|---|---|---|---|---|---|---|---|---|
| Native centromere 9 | Chr9:755,771–762,621 | 6.84 | - | 43.6 | - | - | - | - |
| cen9∆-A | Chr9:785,352–789,247 | 3.87 | 56.6 | 46.1 | Escrt-II complex subunit (VPS25) | CNBG_5690 | 100 | |
| Iron regulator 1 | CNBG_9614 | 14.6 | Last exon | |||||
| cen9∆-B | Chr9:775,164–780,756 | 4.41 | 64.5 | 46.6 | Xylosylphosphotransferase (XPT1) |
CNBG_5687 | 6.9 | |
| Transglycosylase SLT domain-containing protein | CNBG_9613 | 100 | ||||||
| Glutamate synthase (NADPH/NADH) | CNBG_5689 | 33.7 | ||||||
| cen9∆-C | Chr9:775,164–780,756 | 4.41 | 64.5 | 46.6 | Xylosylphosphotransferase (XPT1) |
CNBG_5687 | 6.9 | |
| Transglycosylase SLT domain-containing protein | CNBG_9613 | 100 | ||||||
| Glutamate synthase (NADPH/NADH) | CNBG_5689 | 33.7 | ||||||
| cen9∆-D | Chr9:750,902–755,294 | 4.37 | 63.9 | 41.9 | Hypothetical protein | CNBG_5684 | 92.8 | |
| Derlin-2/3 | CNBG_5685 | 100 | ||||||
| cen9∆-E | Chr9:775,164–780,756 | 5.56 | 81.3 | 50 | Xylosylphosphotransferase (XPT1) |
CNBG_5687 | 6.9 | |
| Transglycosylase SLT domain-containing protein | CNBG_9613 | 100 | Last exon | |||||
| Glutamate synthase (NADPH/NADH) | CNBG_5689 | 33.7 | Last exon | |||||
| cen9∆-F | Chr9:771,614–775,469 | 3.83 | 56.0 | 51.5 | Xylosylphosphotransferase (XPT1) | CNBG_5687 | 100 | |
| Native centromere 10 | Chr10:362,876–369,657 | 6.77 | - | 42.6 | - | - | - | - |
| cen10∆-A | Chr10:115,954–120,422 | 4.46 | 65.9 | 46.9 | CENPC/MIF2 | CNBG_4461 | 88.3 | 1, 2, 3, 4 (only5th is outside) |
| Hypothetical protein | CNBG_4462 | 100 | ||||||
| Chr10:391,090–393,946 | 2.85 | 42.1 | 48.9 | Serine/threonine-protein phosphatase 2A activator 2(RRD2) | CNBG_9459 | 10.6 | Last exon (5th) | |
| Hypothetical protein | CNBG_4366 | 100 | ||||||
| Hypothetical protein | CNBG_4365 | 23.4 | Last exon (3th) | |||||
| cen10∆-B | Chr10:115,954–120,422 | 4.46 | 65.9 | 46.9 | CENPC/MIF2 | CNBG_4461 | 88.3 | 1, 2, 3, 4 (only 5th is outside) |
| Hypothetical protein | CNBG_4462 | 100 | ||||||
| cen10∆-C | Chr10:115,954–120,422 | 4.46 | 65.9 | 46.9 | CENPC/MIF2 | CNBG_4461 | 88.3 | 1, 2, 3, 4 (only 5th is outside) |
| Hypothetical protein | CNBG_4462 | 100 | ||||||
| Chr10:391,090–393,946 | 2.85 | 42.1 | 48.9 | Serine/threonine-protein phosphatase 2A activator 2(RRD2) | CNBG_9459 | 10.6 | Last exon (5th) | |
| Hypothetical protein | CNBG_4366 | 100 | ||||||
| Hypothetical protein | CNBG_4365 | 23.4 | Last exon (3th) | |||||
| cen10∆-D | Chr10:352,648–355,154 | 2.51 | 37.1 | 48 | Ser/Thr protein kinase | CNBG_4379 | 88.4 | |
| cen10∆-E | Chr10:1–4,385 | 4.38 | 64.7 | 53.2 | Hypothetical protein | CNBG_10450 | 100 | |
| Hypothetical protein | CNBG_4495 | 100 | ||||||
| cen10∆-F | Chr10:342,517–345,159 | 2.64 | 39.0 | 45.5 | Hypothetical protein | CNBG_4383 | 18.6 | Last two exons |
| Hypothetical protein | CNBG_10075 | 100 | ||||||
| Hexokinase (HXK1) | CNBG_4382 | 15.3 | Last three exons | |||||
| cen10∆-G | Chr10:378,389–386,366 | 7.97 | 117.7 | 46.5 | High osmolarity signaling protein (SHO1) | CNBG_4373 | 100 | |
| Hypothetical protein | CNBG_4372 | 100 | ||||||
| Hypothetical protein | CNBG_4371 | 100 | ||||||
| Hypothetical protein | CNBG_4370 | 100 | ||||||