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Challenge with SSV9 yields isolated chronically infected S. islandicus strains. Growth of an isolated, chronically infected strain (Δcas6:SSV9.1, solid blue line) and an uninfected isogenic strain (Δcas6 strain, gold line), as monitored by optical density. Throughout the growth curves, SSV9 production (blue dashed line) was monitored in the chronically infected strain by plaque assay. Error bars show mean results ± standard errors of the means (SEM) (n = 3).
