Cryo-EM data collection, refinement and validation statistics
	Class-1 (open) (EMDB-21529) (PDB 6W2S)	Class-2 (closed) (EMDB-21530) (PDB 6W2T)
Data collection and processing
Magnification Voltage (kV) Electron exposure (e–/Å2) Defocus range (μm) Pixel size (Å) Symmetry imposed Initial particle images (no.)	130,000 300 59.55 −1 /– 3 1.06 C1 915,647
Final particle images (no.)	14,257	23,444
Map resolution (Å) FSC threshold	3.3 0.143	3.3 0.143
Map resolution range (Å)	3–8	3–8
Refinement
Initial model used (PDB code)	5A2Q	5A2Q
Model resolution (Å) FSC threshold	3.6 0.5	3.6 0.5
Model resolution range (Å)	3.3–8	3.3–8
Map sharpening B factor (Å2)	−31.94	−43.41
Model composition Non-hydrogen atoms Ligands	106,817 -	109,684 -
B factors (Å2) Protein RNA	92.47 114.1	96.5 117.4
R.m.s. deviations Bond lengths (Å) Bond angles (°)	0.014 1.77	0.014 1.78
Validation MolProbity score Clashscore Poor rotamers (%)	2.12 6.13 1.62	1.99 4.92 1.39
Ramachandran plot Favored (%) Allowed (%) Disallowed (%) RNA validation Angles outliers (%) Sugar puckers outliers (%) Average suit	88.92 98.37 1.63 0.18 2.35 0.442	90.25 98.50 1.50 0.17 2.25 0.428