Video 1. Representative Movie of Opa-llama expression.

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An embryo expressing maternal free EGFP, maternal Histone H2Av-RFP, and zygotic llama-tagged opa is shown here in a time-lapse image that initiates at mitosis 12 and continues through early germband extension. The counter in the upper left corner indicates time (hh:mm) relative to the start of NC14. Top panel shows the opa-llama (EGFP) channel. Middle panel shows His2Av-RFP, which marks nuclei at all times. Bottom panel shows the merge of these two channels. In addition to features of the movie described in the Results section, note the persistence of Opa on mitotic chromatin during zygotic mitoses within the segmental primordium (>1 hr:15 m) compared with EGFP background in head region mitotic domains.