Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | bcdE2 osk166 cic1 tsl1 TlRM9 | This paper | Flybase allele IDs: FBal0001081 FBal0013308 FBal0103875 FBal0017195 FBal0016839 |
Quintuple mutant generated by multiple rounds of meiotic recombination as described. |
| Genetic reagent (D. melanogaster) | opaIIP32 | Jürgens et al., 1984 | Flybase allele ID: FBal0013272 |
|
| Genetic reagent (D. melanogaster) | P(His2Av-EGFP) | BloomingtonDrosophilaStock Center | Flybase allele ID: FBal0183310 |
|
| Genetic reagent (D. melanogaster) | opa-3xMyc | This paper | CRISPR/Cas9 allele | |
| Genetic reagent (D. melanogaster) | opa-EGFP llama | This paper | CRISPR/Cas9 allele | |
| Genetic reagent (D. melanogaster) | Bcd promoter > EGFP | This paper | Transgenic insertion into P(CaryP)attP40 (Flybase transposable element insertion site ID: FBti0114379) | |
| Genetic reagent (D. melanogaster) | alpha Tubulin67c > opa-3xMyc | This paper | Transgenic insertion into P(CaryP)attP40 (Flybase transposable element insertion site ID: FBti0114379) | |
| Antibody | Anti-myc polyclonal antibody (rabbit) | Sigma-Aldrich | Cat #: C3956 | ChIP: 1 ug IF: 1:200 |
| Antibody | Anti-eve monoclonal antibody (mouse) | Developmental Studies Hybridoma Bank | Clone number 2B8 | IF: 1:20 |
| Antibody | Anti-runt polyclonal antibody (guinea pig) | Wieschaus Lab, Princeton University | IF: 1:1000 | |
| Software, algorithm | TrimGalore! | https://github.com/FelixKrueger/TrimGalore | ||
| Software, algorithm | Bowtie2 | Langmead and Salzberg, 2012 | ||
| Software, algorithm | Picard MarkDuplicates | http://broadinstitute.github.io/picard/ | ||
| Software, algorithm | MACS2 | Zhang et al., 2008 | ||
| Software, algorithm | IDR | https://www.encodeproject.org/software/idr/ | ||
| Software, algorithm | Bedtools | https://bedtools.readthedocs.io/en/latest/ | ||
| Software, algorithm | Samtools | http://www.htslib.org/ | ||
| Software, algorithm | GenomicAlignments 1.18.1 | Lawrence et al., 2013 | ||
| Software, algorithm | GenomicRanges 1.34.0 | Lawrence et al., 2013 | ||
| Software, algorithm | DESeq2 1.22.2 | Love et al., 2014 | ||
| Software, algorithm | Gviz 1.26.5 | Hahne and Ivanek, 2016 | ||
| Software, algorithm | Biostrings 2.50.2 | Pagès et al., 2019 |