Figure 4.

N-terminal FANCB variants affect FA core complex assembly. (A) Schematic for protein purifications of human FANCB complexes purified from baculovirus infected insect cells. (B) Coomassie blue–stained sodium dodecyl sulfate–polyacrylamide gel electrophoresis gel of affinity-purified FANCB-FANCL-FAAP100 (BL100) complex and bar graph (n = 2 experiments) indicating relative molar amount of FANCL and FAAP100 copurified with WT of variant Flag-FANCB protein as bait. (C) As in panel B but using full FA core complex. Western blots for FANCA, FANCG, FANCE, and FANCL are shown to identify individual Coomassie-stained bands. Asterisks represent contaminant proteins that bind to Flag-affinity resin.