Fig. 5.

GTS-21 modulates the release of IL-6 (A) and NO (B) induced by LPS in PBMCs of COPD patients and healthy subjects. PBMCs (106 cells/mL) from COPD patients (n = 15) or healthy subjects (n = 6) were cultured with LPS (100 ng/mL) for 24 h or 4 h prior to cells challenging by GTS-21 (10 μmol/l) for 20 h more. The supernatants were harvested and used for IL-6 (A) or nitrites (B) measurement by ELISA and Griess modified method, respectively. Data are expressed as Mean ± SEM (**p < 0.01, ***p < 0.001).