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. 2020 Apr 12;10(12):5514–5526. doi: 10.7150/thno.40520

Figure 5.

Figure 5

Ultrasound stimulation modulates the neuronal excitability in epileptic slices. (A) Representative voltage traces recorded from interneurons of epileptic slices in response to a sequence of sustained currents injection (50, 150, and 250 pA). During ultrasound stimulation, interneurons could activate more action potentials. (B) Double-staining of biocytin-injected neurons with GAD 67 antibody showing a typical morphology of interneuron. Scale bar = 10 μm. (C) Ultrasound stimulation (red) significantly increased the firing frequency of interneurons compared with Before US (black, Student's paired t-test, ** P < 0.01). (D) Representative voltage traces recorded from pyramidal neurons of epileptic slices in response to a sequence of sustained currents injection (150, 250, and 350 pA). Ultrasound stimulation suppressed the firing frequency of pyramidal neurons. (E) Biocytin-injected staining showing a typical morphology of pyramidal neurons. Scale bar = 20 μm. (F) Ultrasound stimulation (red) significantly decreased the firing frequency of pyramidal neurons compared with Before US (black, Student's paired t-test, * P < 0.05; ** P < 0.01).