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. 2020 Mar 16;215(1):89–101. doi: 10.1534/genetics.120.303144

Figure 4.

Figure 4

Testing the interaction between Sry-δ and 32B using biFC. (A) Graph showing the fraction of cells showing biFC of indicated cV-tagged proteins with 32B-nV, normalized to 32B-cV with 32B-nV. A minimum of 50 images were counted per sample per experiment, and results are an average of three biological replicates with error bars showing the SD of the normalized replicates. Results for Sry-δ-cV were variable, but it clearly interacted with 32B-nV. (B) Representative micrographs for the indicated proteins. Nuclei were stained with Hoechst and false-colored red, while Venus is shown in green. All images were acquired using the same settings, but the image shown for delBESS-cV had the green channel enhanced to better show the Venus signal. 32B, Boundary Element-Associated Factor-32B; biFC, biomolecular fluorescence complementation; cV, C-terminal part of Venus; nV, N-terminal part of Venus; delBESS, 32B with the BESS domain deleted; Sry-δ, Serendipity δ.