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Nucleotide-content assay. (a) SEPT9GC, (b) SEPT12G. No nucleotide bound to SEPT9 was detected, indicating that SEPT9GC is purified in its apo form. However, small amounts of GDP were released from the SEPT12G sample, indicating that a fraction of the molecules were purified in the form of a GDP-bound complex. Samples of GTP and GDP [100 and 20 µM of each in (a) and (b) respectively; red lines] were used as markers.
