Table 1.

Structural statistics for the structure of hRpn2-bound hRpn13 at proximal ubiquitin of K48-diubiquitin

	Complex
NMR distance and dihedral constraints
Distance restraints
Total NOE	5,475
Intra-residue	1,591
Inter-residue	3,884
Sequential (|i-j| = 1)	1,022
Non-sequential (|i-j| > 1)	2,542
Intermolecular NOEs	320
hRpn13:hRpn2	224
hRpn13:Proximal Ub	72
Proximal Ub:Distal Ub	24
Hydrogen bonds	100
Intramolecular	97
Intermolecular	3
hRpn13:hRpn2	2
hRpn13:Proximal Ub	1
Total dihedral angle restraints	604
Phi	302
Psi	302
Structure statistics
Violations (mean and SD)
Distance constraints (Å)	0.051 ± 0.001
Dihedral angle constraints (°)	0.370 ± 0.034
Max. dihedral angle violation (> 5 °)	0
Max. distance constraint violation (> 0.5 Å)	0
Deviations from idealized geometry
Bond lengths (Å)	0.003 ± 0.000
Bond angles (°)	0.494 ± 0.014
Impropers (°)	0.350 ± 0.010
Average pairwise root-mean-square deviation** (Å)
Heavy atoms	1.00 ± 0.13
Backbone	0.48 ± 0.09

^**Statistics for 15 lowest energy structures without violations for hRpn13 Pru (K21-N130), bound proximal ubiquitin (M1-L71), and hRpn2 (942–951).