Table 2.

Structural statistics for the structure of hRpn2-bound hRpn13 at distal ubiquitin of K48-diubiquitin

	complex
NMR distance and dihedral constraints
Distance restraints
Total NOE	5,459
Intra-residue	1,591
Inter-residue	3,868
Sequential (|i-j| = 1)	1,022
Non-sequential (|i-j| > 1)	2,542
Intermolecular NOEs	304
hRpn13:hRpn2	224
hRpn13: Distal Ub	56
Proximal Ub:Distal Ub	24
Hydrogen bonds	99
Intramolecular	97
Intermolecular	2
Total dihedral angle restraints	604
Phi	302
Psi	302
Structure statistics
Violations (mean and SD)
Distance constraints (Å)	0.051 ± 0.001
Dihedral angle constraints (°)	0.371 ± 0.052
Max. dihedral angle violation (> 5 °)	0
Max. distance constraint violation (> 0.5 Å)	0
Deviations from idealized geometry
Bond lengths (Å)	0.003 ± 0.000
Bond angles (°)	0.494 ± 0.013
Impropers (°)	0.348 ± 0.007
Average pairwise root-mean-square deviation** (Å)
Heavy atoms	1.07 ± 0.16
Backbone	0.59 ± 0.14

^**Statistics for 15 lowest energy structures without violations for hRpn13 Pru (K21-N130), bound distal ubiquitin (M1-L71), and hRpn2 (942–951).