Table 1.
Intra-assay, inter-assay, and inter-mouse variation of plasmin generation parameters
| Plasma dilution | Variation | Lag time (min) | TtPeak (min) | Velocity (nM/min) | Peak (nM) | EPP (nM × min) |
|---|---|---|---|---|---|---|
| 1:3 | Intraassay (N = 7) | 2.3 ± 0.0 (0.0) | 4.6 ± 0.1 (2.8) | 62.6 ± 1.7 (2.7) | 146.2 ± 3.9 (2.7) | 809 ± 22 (2.7) |
| Interassay (N = 4) | 1.5 ± 0.4 (24.9) | 4.1 ± 0.5 (12.3) | 67.4 ± 8.3 (12.4) | 166.0 ± 11.5 (6.9) | 964 ± 33 (3.6) | |
| Intermouse (N = 6) | 2.4 ± 0.2 (5.9) | 4.8 ± 0.2 (3.5) | 57.2 ± 8.0 (14.0) | 151.3 ± 17.4 (11.5) | 888 ± 82 (9.3) | |
| 1:6 | Intraassay (N = 7) | 2.3 ± 0.0 (0.0) | 4.3 ± 0.0 (0.0) | 51.9 ± 3.2 (6.2) | 103.1 ± 6.4 (6.2) | 444 ± 24 (5.5) |
| Interassay (N = 4) | 1.9 ± 0.3 (16.5) | 3.7 ± 0.5 (13.4) | 49.7 ± 8.0 (16.1) | 115.1 ± 9.6 (8.3) | 586 ± 95 (16.2) | |
| Intermouse (N = 6) | 1.9 ± 0.2 (8.8) | 3.9 ± 0.3 (7.1) | 52.6 ± 4.3 (8.2) | 104.8 ± 11.1 (10.6) | 484 ± 83 (17.0) |
Plasmin generation was performed at 1 pM TF, 4 μM phospholipids, and 1.25 μg/mL rtPA. Mean ± standard deviation (% coefficient of variation).