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. 2020 May 5;8:e9110. doi: 10.7717/peerj.9110

Figure 6. Conditioned medium from M2b macrophages may inhibit apoptosis resistance in PASMCs by deregulating the PI3K/Akt/FoxO3a pathway.

Figure 6

PASMCs were treated with serum-free medium, LY294002 (30 µM), the supernatant of M2b macrophages, or a combination of LY294002 (30 µM) and the supernatant of M2b macrophages for 24 hours. The apoptosis and expression levels of Bcl-2 family proteins and cleaved caspase-9 in different groups were detected. (A, B) Annexin V-PE/7-ADD staining was used to assess the apoptosis of PASMCs (n = 3 for each group). (C–H) Western blot was used to assess the protein expression of Bax, Bcl-2, Bcl-xl, cleaved caspase-9, and GAPDH (n = 4 for each group). Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.