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. Author manuscript; available in PMC: 2020 May 11.
Published in final edited form as: Oncogene. 2008 Dec 8;28(6):792–802. doi: 10.1038/onc.2008.437

Figure 1.

Figure 1

Phosphorylation (activation) of ERM proteins is dynamically regulated during metastatic progression. Metastatic lung nodules were evaluated by immunohistochemistry for expression of phosphorylated ERM and total ezrin. Samples were derived after tail vein injection of K7M2 murine osteosarcoma cells (a–g and j) or MNNG/HOS human osteosarcoma cells (h and k), and from human patient tissue samples (i and l). K7M2 lung metastases were examined at days 5, 15, 20 and 24 after injection (a–g and j). A similar pattern of immunoreactivity for ezrin and phosphorylated ERM were seen in K7M2 pulmonary metastases derived from spontaneous lung metastasis from an appendicular primary tumor in mice (data not shown). Total ezrin and phosphorylated ERM were stained on adjacent lung sections. a and d, Bar = 40 μm; b, c, e and f, bar = 100 μm; g–l, bar = 200 μm.