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. 2020 May 5;9:e56186. doi: 10.7554/eLife.56186

Figure 2. ASI sensory neurons secrete DAF-7/TGFβ to specifically lower the nematode’s peroxide resistance.

(A) Diagram summarizing experimental strategy. (B–D) Peroxide resistance of wild type, daf-7(ok3125), and daf-7(ok3125) with daf-7(+) reintroduced with (B) its endogenous promoter, (C) the ASI-specific str-3 promoter, or (D) the ASJ-specific trx-1 promoter. (E–G) Resistance to 5 mM arsenite, 75 mM paraquat, and 25 mM dithiothreitol (DTT) of wild type and daf-7(ok3125). See also Figure 2—figure supplement 1. Statistical analyses are in Supplementary file 2.

Figure 2.

Figure 2—figure supplement 1. DAF-7 regulates peroxide resistance robustly.

Figure 2—figure supplement 1.

(A–B) The daf-7(e1372) loss-of-function mutation increased peroxide resistance at (A) 25°C, and (B) 20°C. (C) daf-7(ok3125) increased peroxide resistance when C. elegans was fed E. coli HT115(DE3) during development, adulthood, and the survival assay. (D) daf-7(ok3125) and daf-1(m40) increase peroxide resistance when we inhibited formation of the eggshell of fertilized C. elegans embryos with RNAi of egg-5 and did not expose nematodes to FUDR at any point. Statistical analyses are in Supplementary file 2.