Figure 3.

Elevation of HIF-1α inhibits, enhances, or has no effect on SC survival in response to different cell death inducers. SC cultures in 96-well plates were treated with various doses of H₂O₂ (A), camptothecin (B), thapsigargin (C), or tunicamycin (D) and survival assessed at either 3 h (H₂O₂) or 24 h (other inducers) by MTS assay to assess the effects of elevation of HIF in response to oxidative stress, DNA damage, ER Ca⁺⁺ release, and the UPR, respectively; n = 3 independent experiments/condition. Mean ± SEM. *, A, 3.9 μM, p = 0.03; 16.3 μM, p = 0.006; 31.3 μM, p < 0.0001; 62.5 μM, p = 0.03; B, 3 μM, p = 0.002; 12 μM, p = 0.014; D, 1 μM, p = 0.037. Figure Contributions: Caitlin Hill and Jessica Curtin performed the experiments. Caitlin Hill analyzed the data.